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pFUSE-CHIg-hG1e2

Product Unit size Cat. code Docs. Qty. Price

pFUSE-CHIg-hG1e2

Human IgG1

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20 µg

pfuse-hchg1e2
+-
$684

pFUSEss-CHIg-hG1e2

Human IgG1 with IL2ss

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20 µg

pfusess-hchg1e2
+-
$684

pFUSE-CHIg
pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region with an increased serum half-life

 

pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

 

Key features of pFUSE-CHIg-hG1e2

  • Encodes the human IgG1e2 CH constant region (M252Y / S254T / T256E + H433K / N434F mutations)
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin™ in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence

 

Reference:

1. Vaccaro C. et al., 2005.  Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol. 23(10):1283-8.

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Specifications

• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations

pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence

Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
- Increased binding to FcRn
- Increased half-life

Both plasmids are selectable with Zeocin™.

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pFUSE Contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin® (100 mg/ml)

room temperature Product is shipped at room temperature

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